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Ostriches are susceptible to bacterial, fungal and parasitic diseases. One of the most commonstrategies to reduce microbial contamination in animal production systems is to identify microbesources. In this regard, a first critical component for comprehensive farm-to-fork strategies to reducethe burden of foodborne illness is the identification of the pathogenic fungi in foodstuffs with animalsources, and the reduction of human pathogen contamination in food production. This study wascarried out to identify to mycoflora in the ostriches' (Struthio camelus) gastrointestinal tract (GIT),in the northwest of Iran. The samples were taken from different parts of the gut tract, including crop,gizzard, intestine and caecum of 50 ostriches. Atotal of 396 fungal colonies were obtained from GIT.These isolates belonged to 17 genera, and Candida (18.7 %), Aspergillus (16.7 %), Monascus (10.6%), Trichosporon (6.6 %) and Fusarium (6 %) were predominant isolates. Among the Candidaisolates, C. tropicalis was the most predominant isolates following by C. albicans, C. glabrata andC. krusei. Aspergillus spp. and Monascus ruber were predominant isolates among the mould fungi.
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